Protein Marker Related Questions

 

Yes, SMOBIO prestained protein markers can be used with silver staining. However, due to the dye modifications used for pre-staining, the visibility of marker bands may vary depending on the staining conditions. To enhance band clarity, we recommend the following:

  • Choose an appropriate silver staining protocol:
    For improved visualization of low molecular weight (low-MW) protein bands, protocols such as the one by Morrissey J.H. are generally more effective. However, this may lead to increased background staining—be sure to optimize the development time accordingly. View reference: https://doi.org/10.1016/0003-2697(81)90783-1

    If a cleaner background is preferred, the Alphalyse protocol can be used instead, although it may result in weaker staining of low-MW bands. View protocol: https://alphalyse.com/wp-content/uploads/2025/01/Silver-staining-protocol.pdf

  • Optimize staining conditions:
    Adjust parameters such as silver nitrate concentration, development time, or fixative composition to balance sensitivity and background.

  • Include an additional gel lane for comparison:
    If protein detection is critical, consider loading a separate lane with a sample of known protein concentration as a visual reference, especially for small bands.